Depletion of endogenous KAP1 expression by RNAi stimulates p53 transcriptional activity, sensitizes p53 response to DNA damage, and increases apoptosis. p53 was determined by Ni-NTA purification of proteins conjugated to His6-ubiquitin and p53 Western blot. As expected, ubiquitination of p53 was stimulated by MDM2, and expression of KAP1 further enhanced p53 ubiquitination level in an … Continue reading Depletion of endogenous KAP1 expression by RNAi stimulates p53 transcriptional activity, sensitizes p53 response to DNA damage, and increases apoptosis